SAI's Alumina for chromatography has contributed to the continued growing use of this material, in a diverse number of applications. Indeed, the major reasons for this growth has been our contribution to the standardization of the manufacturing process resulting in standardized grades of Alumina with very well controlled and defined:
  • Activity (according to Brockmann):
        Super Activity I
        Standard (Std) Activity I-IV
  • pH: Acid(A) Basic(B) Neutral(N)
  • Stepless Deactivation behavior
  • Constant Deactivation behavior
  • Controlled Chromatographic Parameters
  • Controlled Surface Area, Porosity

Scientific Adsorbents' Aluminas
Analytical, Prep LC, Sample Processing

Super I
Super Activity I Aluminas are as unique as SAI's products. They demonstrate approximately twice the capacity as compared to Standard Activity I. Surface modifications available are "A" (Acid), "B" (Basic), and "N" (Neutral). Super Activity I Aluminas constitute the starting material for the Scientific Adsorbent's line of Aluminas. Therefore, it is easy to change between various modes of chromatography. A special feature of Super Activity I is absolutely constant deactivation behavior which is valid for the deactivation process as well as when in contact with the chromatographic solvent.

CAT# Description
4601-05 ALUMINA A, SUPER I, 50-200u, 500g
04601-1 ALUMINA A, SUPER I, 50-200u, 1kg
04601-5 ALUMINA A, SUPER I, 50-200u, 5kg
04601-50 ALUMINA A, SUPER I, 50-200u, 50kg
04577-05 ALUMINA B, SUPER I, 50-200u, 500g
04577-1 ALUMINA B, SUPER I, 50-200u, 1kg
04577-5 ALUMINA B, SUPER I, 50-200u, 5kg
04577-50 ALUMINA B, SUPER I, 50-200u, 50kg
04589-05 ALUMINA N, SUPER I, 50-200u, 500g
04589-1 ALUMINA N, SUPER I, 50-200u, 1kg
04589-5 ALUMINA N, SUPER I, 50-200u, 5kg
04589-50 ALUMINA N, SUPER I, 50-200u, 50kg

Standard Activity I
Alumina Standard Activity I (Act I) is available with various surface modifications, A, B, and N to facilitate the separation of a wide range of compounds. In addition to pH, the activity of the surface of Alumina can mediate the separation. It is simple to adjust the activity by adjusting the water content of the material. (Alternatively, other polar media can replace the water.)

  • Use high activity Alumina (Std Act I, Super Act I) for the separation of polar samples in nonpolar solvent systems... and for the purification of solvents

  • Use lower activity Alumina for less polar samples

    CAT# Description
    02159-05 ALUMINA A, ACT I, 50-200u, 500g
    02159-1 ALUMINA A, ACT I, 50-200u, 1kg
    02159-5 ALUMINA A, ACT I, 50-200u, 5kg
    02159-50 ALUMINA A, ACT I, 50-200u, 50kg
    02078-05 ALUMINA B, ACT I, 50-200u, 500g
    02078-1 ALUMINA B, ACT I, 50-200u, 1kg
    02078-5 ALUMINA B, ACT I, 50-200u, 5kg
    02078-50 ALUMINA B, ACT I, 50-200u, 50kg
    02135-05 ALUMINA N, ACT I, 50-200u, 500g
    02135-1 ALUMINA N, ACT I, 50-200u, 1kg
    02135-5 ALUMINA N, ACT I, 50-200u, 5kg
    02135-50 ALUMINA N, ACT I, 50-200u, 50 kg

DCC Alumina
DCC - Dry Column Chromatography is a versatile Prep LC method that bridges the gap between analytical TLC and preparative column chromatography.  See Silica DCC for detailed procedure.

CAT# Description
04514-05 ALUMINA DCC, 63-200u, 500g
04514-5 ALUMINA DCC, 63-200u, 5kg
04514-50 ALUMINA DCC, 63-200u, 50kg

"Flash" Alumina
Flash Chromatography is a rapid Prep LC technique that facilitates the separation of 0.1 - 10 g of material via simple economical laboratory protocols.

CAT# Description
02061-05 ALUMINA N, 40 MICRONS(FLASH), 32-63u, 500g
02061-1 ALUMINA N, 40 MICRONS(FLASH), 32-63u, 1kg
02061-5 ALUMINA N, 40 MICRONS(FLASH), 32-63u, 5kg
02062-05 ALUMINA N, ACTIVE, 40 MICRONS(FLASH), 32-63u, 500g

Activity II, III
Alumina II - III sorbents are economical adsorbents of medium activity. Use this material for general purpose scouting and in cases where the use of carbon black is precluded due to its organic nature. Also, use Alumina II - III as a replacement for organic/polymeric ion exchangers, especially when it is necessary to overcome temperature and radiation cleavage problems.

CAT# Description
04694-05 ALUMINA ACT II-III, 50-200u, 500g
04694-5 ALUMINA ACT II-III, 50-200u, 5kg
04694-50 ALUMINA ACT II-III, 50-200u, 50kg

Alumina for HPLC
The following Catalog #s are recommended:

CAT# Description
02142 ALUMINA N, 5u, 10g
02143 ALUMINA N, 5u, 100g
02148 ALUMINA N, 10u, 10g
02149 ALUMINA N, 10u, 100g
02151 ALUMINA N, 15u, 10g
02152 ALUMINA N, 15u, 100g
02156 ALUMINA N, 25u, 10g
02157 ALUMINA N, 25u, 100g
02063 ALUMINA A, ACTIVE, 18-32u, 100g
02065 ALUMINA B, ACTIVE, 18-32u, 100g
02058 ALUMINA N, ACTIVE, 18-32u, 10g
02059 ALUMINA N, ACTIVE, 18-32u, 100g

Alumina Oxides for Thin-Layer Chromatography
The following Catalog #s are recommended:

CAT# Description
04347-1 ALUMINA A-TLC, 1kg
04347-50 ALUMINA A-TLC, 50kg
04341-1 ALUMINA B-TLC, 1kg
04341-50 ALUMINA B-TLC, 50kg
04344-1 ALUMINA N-TLC, 1kg
04344-50 ALUMINA N-TLC, 50kg
04413-1 ALUMINA G-TLC, 1kg
04413-50 ALUMINA G-TLC, 50kg

Deactivation Protocols

SAI's Aluminas are unique products, i.e. Super I, Std Act I, etc. High activity Alumina can be used for polar samples in nonpolar solvents, and for the purification of solvents. Lower activities of Alumina can readily be obtained by the addition of polar media, especially water. Thus each problem can be discretely resolved via the adjustment of each sorption system.

Special Features
Super I Aluminas show an approximate double capacity as compared to Activity I. Super I does not have to be deactivated in steps. By following the appropriate deactivation curves, deactivation can be achieved in minute increments.

Deactivation behavior
By following the procedures below, it is relatively easy to obtain the desired activity.

Deactivation Behavior - Alumina
Activity Grade
Alumina Type Super I I II III IV V  
Super I - A,B,N 0 1 4 7 10 19 % H2O Added
Std Act I - A,B,N NA 0 3 6 10 15 % H2O Added
A = Acid, B = Base, N = Neutral

Deactivation Procedure(s)
The % water addition shown above is based upon weight / weight relationships; these relationships are critical and any deviation will/could result in obtaining improper activities.

To reproduce the desired activity, weigh an appropriate amount of Alumina into a glass bottle. Add the appropriate weight of water to the Alumina and close the bottle with a stopper. For example, 97 g of Alumina + 3 g H2O = 3% water addition.

Shake well until all lumps disappear. Wait until the mixture has cooled to room temperature. Keep the container closed so that equilibrium conditions remain constant.

Application: Typical Chromatography Uses of Alumina
Application Recommended Alumina
Isolation from ergot-, opium-, rauwolfia-, and other alkaloids
Basic, medium activity, Specialty
Isolation, purification
Removal of terpenes
Basic, Neutral
Isolation of active substances
Basic, Neutral, Acid
Isolation of digitalis-, strophantus-, glycosides, etc.
from organic solvents
Basic, highly active
Isolation and purification of synthetic products, of ketosteroids from neutral materials, etc.
for analytical and technical purposes
Basic, highly active
Clarification of fatty oils, separation of fatty acids
Removal from solvents, transformer oils
Alumina "C"
from injectable solutions and infusions
Alumina "P"

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